Cat. No.
MABL-277
Application
functional assay, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
LM5
From
Recombinant Antibody
Specificity
This antibody is specific for beta-1,4-galactan. It specifically recognized more than 3 contiguous units of beta-1,4-galactan. Beta-1,4-galactan is expressed prefominantly in the cell wall.
Alternative Names
UniProt
Immunogen
The rat version of this antibody was raised by immunizing a wister rat with a polysaccharide.
Application Notes
To characterize this antibody, a competitive ELISA was performed on saccharides using the original rat version of this antibody. Furthermore, an immunoblot was performed on citrus pectin, apple pectin, TG1, TG2a, TG2b, GALCBSA, BSA, lupin galactan, gum arabic, polygalacturonic acid, larchwood arabinogalactan, and locust bean gum using this antibody. Finally, tomato pericarp was immunogold labelled using this antibody (Jones et al, 1997; pmid:12223681). To map the epitope of this antibody, an ELISA was performed on different lengths of beta-1,4-galactan and beta-1,4-galactan with different side groups. It showed the greatest binding efficiency to 4 units of beta-1,4-galactan (Andersen et al, 2016; pmid:27855335). While creating and characterizing an antibody against (1→5)-α-l-arabinan, the rat version of this antibody was used as a control in an ELISA. Furthermore, a immunoblot was performed on surfaces of lemons using this antibody (Willats et al, 1998, pmid:9675359). Pea cotyledons were stained for immunofluorescence using the rat version of this antibody to study plant cell wall architecture.
Furthermore, immunogold assay was performed on pea cotyledons using this antibody (McCartney et al, 2000; pmid:10792826).
Antibody First Published
Jones et al. Localization of Pectic Galactan in Tomato Cell Walls Using a Monoclonal Antibody Specific to (1[->]4)-[beta]-D-Galactan Plant Physiol. 1997 Apr;113(4)1405-1412. PMID:12223681
Note on publication
Antibodies againt saccharides were created and characterized.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
