Cat. No.
MABL-274
Application
IP, WB, FC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
ham151-149
From
Recombinant Antibody
Specificity
The antibody reacts with mouse Bim. Mouse Bim is a 19-24 kDa pro-apoptotic protein (Bcl-2 family) which regulates immunological responses.
Alternative Names
Bcl-2-like protein 11; Bcl2-L-11; Bcl2-interacting mediator of cell death
UniProt
O54918
Immunogen
The original antibody was generated from a hamster immunized with a complex of mouse Bim and Bcl-XL produced in insect cells.
Application Notes
The antibody reacted in Western blots and by immunofluorescence with permeabilized B and T cells from Bim +/+ mice (Oliver et al., 2004; PMID: 15520248). T cells were analyzed for levels of Bcl-2 and Bim protein by staining of permeabilized cells with the antibody (Jorgensen et al., 2007; PMID: 17785775). The antibody was used for flow cytometry analysis (Kashiwakura et al., 2013; PMID: 23278598).
Antibody First Published
Oliver et al. Loss of Bim Allows Precursor B Cell Survival But Not Precursor B Cell Differentiation in the Absence of Interleukin 7. J Exp Med. 2004 Nov 1;200(9)1179-87. PMID15520248
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
