Key features and details | |
Cat. No. | MABL-758 |
Name | Anti-CD19 mAbs |
Clone No. | AFD- 6D5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody recognises mouse CD19, which is a transmembrane glycoprotein that regulates B lymphocyte development, activation, and differentiation. This clone 6D5 recognizes the same epitope as the published clone 1D3. |
Alternative Name | Leu12; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19 T-cell surface antigen Leu-12; CD19; B4 |
UniProt | P15391 |
Immunogen | This antibody was raised by immunising rats with mouse CD19-expressing K562 human erythroleukemia cells. |
Application Notes | 6D5 antibody has been extensively used to identify B cells in various experimental arrangements. It can be efficiently utilised in the localisation of B cells in various tissues. For instance, one group stained murine spleen tissue cryosections to visualise lymphomas in azathioprine-treated mice (Chalastanis et al., 2010). This antibody has also been widely used in flow cytometry. One group utilised in the flow cytometric identification of B cells in a study investigating the relation of microglia depletion and MPTP neurotoxicity (Yang et al., 2018). |
Antibody First Published | Krop et al. Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42. PMID:8566073 |
Note on publication | This article describes the method of generation of antibodies like 6D5. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
