Key features and details | |
Cat. No. | MABL-736 |
Name | Anti-CD16 mAbs |
Clone No. | AFD- CB-16 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, FC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the CD16 protein. |
Alternative Name | FCGR3A; CD16-II; CD16a; FcR-10; CD16a antigen; IgG Fc receptor III-2; Fc-gamma RIII-alpha; Low affinity immunoglobulin gamma Fc region receptor III-A |
UniProt | P08637 |
Immunogen | The original antibody was raised by immunizing a BALB/c mouse with CD16. |
Application Notes | The original version of this antibody was used for flow cytometry on human neutrophils (Bankey et al., 2011; PMID:20123111) on human bone marrow cells (Allan et al., 2010; PMID:20540115), on PBMCs (Ingersoll et al., 2010; PMID:19965649), on differentiated pluripotent stem cells (Rönn et al., 2015; PMID:25680478), and on whole blood leukocytes (Veras et al., 2020; PMID:32926098).This antibody was used for a whole blood intracellular cytokine assay on NK cells, it showed a very strong response to the antibody (Violari et al., 2019; PMID：30679439). This antibody was used to stimulate NK cells, and its ability to trigger activation and expansion of peripheral blood NK cells (Kim et al., 2023; PMID:37302991). |
Antibody First Published | Ingersoll et al. Comparison of gene expression profiles between human and mouse monocyte subsets Blood. 2010 Jan 21;115(3):e10-9. PMID:19965649 |
Note on publication | The original paper studied the similarity between the two main monocyte subsets in human and mouse blood. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
