Key features and details | |
Cat. No. | MABL-724 |
Name | Anti-CD147 mAbs |
Clone No. | AFD- OX-47 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, WB, FC, IHC-Fr |
Species Reactivity | Rat |
Basic Information | |
Specificity | This antibody is specific for rat CD147, which has a broad distribution; OX-47 labels T and B cell blasts, peritoneal macrophages, the endothelium of all except large vessels and kidney tubules, but does not label TDL and thymocytes. |
Alternative Name | Basigin; BSG; extracellular matrix metalloproteinase inducer; EMMPRIN; Glycoprotein CE9; OX-47 antigen |
UniProt | P26453 |
Immunogen | Mice immunized with rat T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells. |
Application Notes | This antibody binds mouse CD147, and has been used in FC and IHC-fr analysis to characterise CD147 and detect the cell types on which it is expressed, indicating that CD147 is a lymphocyte activation marker (Paterson, 1987). This antibody failed to give immunoprecipitates from radiolabelled activated T cells. |
Antibody First Published | Paterson et al. Antigens of activated rat T lymphocytes including a molecule of 50,000 M r detected only on CD4 positive T blasts Molecular Immunology, 1987, Vol.24(12), pp.1281- 1290 PMID；2828930 |
Note on publication | Describes the original use of this antibody as part of FC and IP studies to identify new markers for activated T cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
