Anti-CD14 mAbs

Cat. No.

MABL-715

Name

Anti-CD14 mAbs

Clone No.

AFD- MiL2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, FC, IF

Species Reactivity

Pig, Human

Basic Information

Specificity

This antibody is specific for porcine CD14.

Alternative Name

Monocyte antigen; Monocyte differentiation   antigen CD14; Myeloid cell- specific leucine-rich glycoprotein

UniProt

P08571

Immunogen

The original antibody was raised by   immunizing a mouse with porcine CD14.

Application Notes

Western blot was performed using this   antibody on proteins (US20170107294). This antibody was used for flow   cytometery on fresh human or porcine whole blood (US20170107294) (Thorgersen   et al, 2009; pmid:19047409). The biological effect of this antibody on the   inflammatory response of piglets was tested (US20170107294). ELISA was   performed on C1q and human FcγRs using this antibody (US20170107294).   Immunofluorescence was performed on both PBMC and whole blood samples using   this antibody (Zelnickova et al, 2007; pmid:17720184). Immunofluorescent   staining was performed on cardiac and diaphragmatic lung lobes using this   antibody (Gucht et al, 2005; pmid:15802956).

Antibody First Published

Gucht et al. Porcine reproductive and   respiratory syndrome virus infection increases CD14 expression and   lipopolysaccharide-binding protein in the lungs of pigs. Viral   Immunol.2005;18(1):116-26. PMID:15802956

Note on publication

The levels of CD14 expression and   LPS-binding protein (LBP) in the lungs of pigs throughout a PRRSV infection   were quantified.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.