Anti-GluCl mAbs

Cat. No.

MABL-046

Name

Anti-GluCl mAbs

Clone No.

AFD-GluCl

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallisation, WB

Species Reactivity

C. elegans

Basic Information

Specificity

The antibody is specific for C. elegans GluCl. Customers report no detection of Drosophila GluCl by IHC.

Alternative Name

Glutamate-gated chloride channel

UniProt

G5EBR3

Immunogen

The antibody was raised against GluCl using standard methods.

Application Notes

The specificity of the antibody for properly folded pentameric GluCl has been assayed in Western blot and FSEC. A Fab fragment of this antibody was used to determine the structure of C. elegans GluCl by X-ray crystallography. Binding of the Fab to GluCl does not affect glutamate- or ivermectin- induced activity.

Antibody First Published

Hibbs et al. Principles of   activation and permeation in an anion-selective Cys-loop receptor. Nature. 2011 Jun 2;474(7349):54-60 PMID:21572436

Note on publication

Describes the crystallisation of GluCl in complex with ivermectin and Fab.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.