Cat. No.
MABL-3703
Application
antagonist, functional assay, in vivo, inhibition, neutralizing, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
Farletuzumab (MORAb-003; M3)
From
Recombinant Antibody
Specificity
This antibody binds human folate receptor alpha (FOLR1). FOLR1 binds folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells by receptor mediated endocytosis. It is required for normal embryonic development and normal cell proliferation. The human folate receptor-alpha (FOLR1) is overexpressed in ovarian cancer but largely absent in normal tissue. It is hypothesized that the presence of elevated levels of FRα can be correlated with the propagation rate and phenotype of the tumors.
Alternative Names
FRA; Frα; Fr-α; FBP; LK26; Folate receptor 1; Folate receptor alpha; FR- alpha; Adult folate-binding protein; Ovarian tumor-associated antigen MOv18; KB cells FBP; Folate receptor adult; MOv18
UniProt
P15328
Immunogen
The original parental mouse antibody was generated by immunizing (BALB/c X C57BL/6) F1 mice by intraperitoneal injection containing cultured LU-75(c) choriocarcinoma cells to produce hybridomas that generated the antibody LK26. Later on the Farletuzumab was generated by CDR grafting technique by taking CDRs of mouse parental clone LK26 and grafting them on human framework regions.
Application Notes
This antibody was derived from the optimization of mouse parental LK26 antibody using a whole-cell genetic evolution platform. This antibody possesses growth-inhibitory activity on cells overexpressing FR-alpha. It elicited robust antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) in vitro and inhibited the growth of human ovarian tumor xenografts in nude mice. Immunohistochemistry studies determined that MORAb-003 infrequently stained the tubular epithelium, epithelium of the fallopian tube, and duct epithelium of the pancreas in both normal human and cynomolgus monkey tissues. Cynomolgus monkey was therefore considered an appropriate toxicology model (Ebel et al., 2007; PMID: 17346028). This antibody was also found to antagonize the activity of human FRα, resulting in the loss of growth advantage conferred by overexpression of FRα under conditions that bracket physiological (10–100 nM) folate concentrations (Routhier et al., 2006; DOI: 10.1200/jco.2006.24.18_suppl.10108).
Antibody First Published
Ebel et al. Preclinical evaluation of MORAb-003, a humanized monoclonal antibody antagonizing folate receptor-alpha. Cancer Immun. 2007; 7:6. PMID:17346028
Note on publication
Describes the generation of a humanized version of an ani-FOLR1 antibody and tests its ADCC and CDC activity in vitro and in vivo for therapeutic applications.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
