Cat. No.
MAK-0118
Product Name
Rabbit IgM
Storage
4°C
Species
Rabbit
Instructions
The Rabbit IgM ELISA kit is intended for measurement of IgM in serum and plasma.
Introduction
The Rabbit IgM ELISA kit is intended for measurement of IgM in serum and plasma. Goat anti-rabbit IgM heavy chain antibodies are used for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-rabbit IgM heavy chain antibodies are used for detection. Normal rabbit IgM levels are in the range of 0.2-3.0 mg/ml in serum or plasma.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared Rabbit IgM standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgM molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgM is proportional to the optical density of the test sample and is derived from a standard curve.
