Cat. No.
MAK-0110
Product Name
Guinea Pig IgG
Storage
2-8°C
Species
Guinea Pig
Instructions
The guinea pig IgG ELISA kit is designed for measurement of IgG in guinea pig serum or plasma.
Introduction
The guinea pig IgG ELISA kit is designed for measurement of IgG in guinea pig serum or plasma. The assay uses goat anti-guinea pig IgG for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-guinea pig IgG antibodies for detection. The immobilization and detection antibodies are cross-adsorbed with guinea pig IgA and IgM, ensuring specificity for IgG.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared guinea pig IgG standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgG molecules are thus sandwiched between the immobilization and detection antibodies.
The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgG is proportional to the optical density of the test sample and is derived from a standard curve.
