Cat. No.
MAK-0102
Product Name
Dog IgE
Storage
4°C
Species
Dog
Instructions
The dog IgE ELISA kit is designed for measurement of IgE in dog serum or plasma.
Introduction
The dog IgE ELISA kit is designed for measurement of IgE in dog serum or plasma. The assay uses a monoclonal dog IgE antibody (IGE-4-4D1) for solid phase (microtiter wells) immobilization, and a horseradish peroxidase (HRP) conjugated dog IgE monoclonal antibody (IGE-4-13H5) for detection.
Principle Of The Assay
Samples are diluted and incubated in microtiter wells for 45 minutes alongside dog IgE standards. The wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgE molecules, if present, are sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP conjugate. TMB is added and incubated for 20 minutes. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow and absorbance is measured at 450 nm. The concentration of IgE is derived from a standard curve.
