Cat. No.
MAK-0098
Product Name
Monkey IgG1
Storage
4°C
Species
Monkey
Instructions
This ELISA kit is designed for measurement of IgG1 in old world monkey serum or plasma.
Introduction
This ELISA kit is designed for measurement of IgG1 in old world monkey serum or plasma. The assay uses monoclonal anti-monkey IgG for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated mouse monoclonal anti- monkey IgG1 antibody for detection. The ELISA does not recognize human IgG. Cross-reactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside monkey IgG1 standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgG1 molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP conjugate. TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgG1 is proportional to the optical density of the test sample and is derived from a standard curve.
