Cat. No.
MAK-0089
Product Name
Rat IgG
Storage
4°C
Species
Rat
Instructions
The rat IgG ELISA kit is designed for measurement of IgG in rat serum or plasma.
Introduction
The rat IgG ELISA kit is designed for measurement of IgG in rat serum or plasma. The assay uses goat anti-rat IgG for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-rat IgG antibodies for detection. Both capture and detection antibodies were cross absorbed on rat IgM and IgA agarose columns, thereby ensuring specificity for IgG. Crossreactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Test samples are diluted and incubated in the anti-rat IgG coated microtiter wells for 45 minutes alongside rat IgG standards. The microtiter wells are subsequently washed, and anti-rat IgG-HRP conjugate is added and incubated for 45 minutes. IgG molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRPlabeled antibodies, and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgG is proportional to the optical density of the test sample and is derived from a standard curve.
