Cat. No.
MAK-0083
Product Name
Mouse IgG2a
Storage
4°C
Species
Mouse
Instructions
The mouse IgG2a ELISA kit is designed for measurement of IgG2a in mouse serum or plasma.
Introduction
The mouse IgG2a ELISA kit is designed for measurement of IgG2a in mouse serum or plasma. The assay uses goat anti-mouse IgG for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-mouse IgG2a antibodies for detection. The capture antibodies were cross-absorbed on mouse IgM and IgA agarose, and the detection antibodies were cross adsorbed on mouse IgM, IgA, IgG1, IgG2b and IgG3 agarose thereby ensuring specificity for IgG2a. Cross-reactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared mouse IgG2a standards. The microtiter wells are subsequently washed. HRP conjugate is added and incubated for 30 minutes. IgG2a molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies. TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow. Optical density is measured spectrophotometrically at 450 nm. The concentration of IgG2a is proportional to the optical density of the test sample and is derived from a standard curve.
