Cat. No.
MAK-0031
Product Name
Human Anti-KLH IgM
Storage
4°C
Species
Human
Instructions
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Introduction
Keyhole limpet hemocyanin (KLH) is a potent immunostimulant used in research studies and clinical applications. Determination of anti-KLH antibody levels allows assessment of immune system regulation. This kit allows quantitative measurement of anti-KLH IgM levels in serum, plasma, and other fluids.
Principle Of The Assay
The assay uses KLH as capture reagent coated on microtiter wells, and horseradish peroxidase (HRP) conjugated anti-human IgM for detection. Standards and diluted samples are incubated in microtiter
wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. Anti-KLH IgM molecules are thus sandwiched between immobilized KLH and the HRP conjugate. The wells are then washed to remove unbound HRP-labeled antibodies. TMB is added and incubated for 20
minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of anti-KLH IgM is proportional to the absorbance and is derived from a standard curve.
