Name
NT5M Human
Cat. No.
MAG-3455
Tag/Conjugates
His
Source
Escherichia Coli.
Shipped
Shipped with Ice Packs
Description
NT5M Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 218 amino acids (32-228 a.a.) and having a molecular mass of 25.1kDa. NT5M is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Introduction
NT5M is a member of the 5'(3')-deoxyribonucleotidase family. NT5M localizes to the mitochondrial matrix. The NT5M enzyme dephosphorylates the 5'- and 2'(3')-phosphates of uracil and thymine deoxyribonucleotides. The NT5M gene is located on chromosome 17 p11.2 in the critical region deleted in the Smith-Magenis syndrome (SMS), a genetic disease of unidentified etiology.
Purification Method
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Physical Appearance
Sterile Filtered clear solution.
Formulation
NT5M protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Purity
Greater than 95% as determined by SDS-PAGE.
Biological Activity
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Safety Data Sheet
SDS
Usage
Mabioways products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Synonyms
5'(3')-deoxyribonucleotidase mitochondrial, 5',3'-nucleotidase, mitochondrial, Deoxy-5'-nucleotidase 2, dNT-2, NT5M, DNT2.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH M GGRALRVLV DMDGVLADFE GGFLRKFRAR FPDQPFIALE DRRGFWVSEQ YGRLRPGLSE KAISIWESKN FFFELEPLPG AVEAVKEMAS LQNTDVFICT SPIKMFKYCP YEKYAWVEKY FGPDFLEQIV LTRDKTVVSA DLLIDDRPDI TGAEPTPSWE HVLFTACHNQ HLQLQPPRRR LHSWADDWKA ILDSKRPC.
Background
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