Cat. No.
MABL-3709
Application
therapeutic
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
SB-240563 (Mepolizumab)
From
Recombinant Antibody
Specificity
This antibody recognizes and binds human interleukin-5, a key cytokine responsible for the differentiation, maturation, recruitment and activation of human eosinophils. This antibody does not cross react with mouse IL-5.
Alternative Names
IL5; Interleukin-5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF; h2B6; humanized 2B6
UniProt
P05113
Immunogen
The original antibody was generated by grafting of CDRs from mouse parental antibody 2B6 onto human framework regions. The parental mouse antibody 2B6 was generated by immunizing mice with recombinant human IL-5.
Application Notes
The pharmacokinetics of this antibody was investigated after intravenous and subcutaneous administration to cynomolgus monkeys. Suppression of eosinophil count was observed after multiple monthly administrations of this antibody to monkeys (PMID: 10565825). Pre-clinical efficacy and safety studies in cynomolgus monkeys demonstrated that chronic antagonism of IL-5 by mepolizumab is safe and has the potential to be beneficial therapy for chronic inflammatory respiratory diseases (PMID: 11496242). In a double-blind randomized placebo-controlled trial, the effects of treatment on responses to inhaled allergen challenge, sputum eosinophils, and airway hyper-responsiveness to histamine were measured at weeks 1 and 4 after a single intravenous infusion of this antibody. This antibody lowered the mean blood eosinophil count in placebo group and prevented the blood eosinophilia that follows allergen challenge. It also significantly reduced sputum eosinophilia seen after inhaled allergen challenge. It was further reported that this antibody had no significant effect to IL-5 on the late asthmatic response or on airway hyperresponsiveness to histamine (PMID: 11191542).
Antibody First Published
Zia-Amirhosseini et al. Pharmacokinetics and pharmacodynamics of SB- 240563, a humanized monoclonal antibody directed to human interleukin-5, in monkeys. J Pharmacol Exp Ther. 1999 Dec;291(3):1060-7. PMID:10565825
Note on publication
This paper describes the pharmacokinetics and pharmacodynamics of a humanized antibody SB-240563 against human IL-5.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
