Cat. No.
MABL-3672
Application
inhibit, neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
CAT-213 (Bertilimumab; iCo-008)
From
Recombinant Antibody
Specificity
This antibody binds human eotaxin (CCL11). This CC chemokine directly promotes the accumulation of eosinophils in response to the presence of allergens, which is a prominent feature of allergic inflammatory reactions. It binds to CCR3.
Alternative Names
SCYA11; Eotaxin; C-C motif chemokine 11; Eosinophil chemotactic protein; Small-inducible cytokine A11; 3G3
UniProt
P51671
Immunogen
The original scFv antibody 3G3 was isolated from a human scFv phage display library after three rounds of panning against human eotaxin1. Optimization of 3G3 was conducted by heavy chain CDR3 randomization with point mutagenesis and light chain shuffling to obtain CAT-212 (scFv). Later on the sequence of CAT-212 was used to generate human IgG4 version of the antibody, which was called CAT-213.
Application Notes
The binding specificity of this antibody for eotaxin 1 was demonstrated using ELISA. The scFv version of this antibody binds human eotaxin1 with a dissociation constant (KD) of 80.4 pM. This antibody demonstrated inhibition of eotaxin1 stimulated chemotaxis of L1.2-hCCR3 cells in vitro. This antibody also inhibited eotaxin1-induced eosinophil shape change with an IC50 of 0.71 nM. In vivo administration of human eotaxin1 to the air pouch of ovalbumin-sensitized mice treated with IL-5 caused a significant dose-related increase in the recruitment of leukocytes to the pouch after 6 h (PMID: 16973884). A paper studied the contribution of eotaxin to chemotactic activity was studied by using a high-affinity neutralizing human anti-eotaxin antibody, CAT213. It was reported that this antibody significantly inhibited chemotactic activity of eosinophils in all asthmatic groups (PMID: 15001461)
Antibody First Published
Main et al. A potent human anti-eotaxin1 antibody, CAT-213: isolation by phage display and in vitro and in vivo efficacy. J Pharmacol Exp Ther. 2006 Dec;319(3):1395-404. PMID:16973884
Note on publication
This paper describes the generation, in vitro and in vivo characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
