Cat. No.
MAK-0150
Product Name
Rabbit CRP
Storage
4°C
Species
Rabbit
Introduction
CRP is an acute phase protein in rabbits that is elevated in serum because of injury, infection, or disease; CRP levels can increase several hundred-fold. Measurement of CRP therefore provides a convenient marker of inflammation and disease in rabbits.
Principle Of The Assay
The assay uses affinity purified rabbit CRP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated rabbit CRP antibodies for detection. The antibodies were generated in chickens using pure rabbit CRP as immunogen and affinity purified on rabbit CRP agarose. In the assay, standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in CRP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If CRP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of CRP is proportional to absorbance and is derived
from a standard curve.
