Cat. No.
MAK-0141
Product Name
Pig AGP
Storage
2-8°C
Species
Pig
Introduction
AGP is a positive acute phase protein in most species. However, Heegard et al., reported that AGP behaves as a negative acute phase reactant in pigs. In contrast, Itoh et al., found elevated levels during inflammatory diseases.
Principle Of The Assay
The assay uses affinity purified pig AGP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated pig AGP antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in AGP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and
TMB is added and incubated for 20 minutes. If AGP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow, and absorbance is measured at 450 nm. The concentration of AGP is proportional to absorbance and is derived from a standard curve.
