Cat. No.
MABL-3414
Application
antagonist, functional assay, Block, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
AFD-STLM208 (ST2H257)
From
Recombinant Antibody
Specificity
This antibody specifically binds an epitope in domain I of the human ST2L and cross reacts with cynomolgus monkey ST2L. ST2L a transmembrane receptor that belongs to the IL-1 receptor family which is expressed on the cell surface of a wide variety of immune cells including T cell, NK/NKT cells, basophils, eosinophils, mast cells and the newly-described non-B/non-T innate lymphoid type 2 cells, nuocytes, and natural helper cells. ST2L expression is also inducible on dendritic cells (DCs), macrophages, and neutrophils. ST2L is able to downregulate the responsiveness of Toll-like Receptors TLR2, TLR4, and TLR9, but also induce type 2 cytokine release via activation by its ligand IL-33 and association with accessory protein IL-1RAcP.
Alternative Names
ST2; IL1RL1; IL-1RL1; DER4; T1; IL-33Rα; Interleukin-1 receptor-like 1; Protein ST2; Growth Stimulation Expressed Gene 2; suppression of tumorigenicity 2; ST2F6; ST2F14
UniProt
Q01638
Immunogen
The original antibody was isolated from a human Fab phage display library by panning against chimeric HHM-ST2L construct with the biotinylated antigen captured on streptavidin-coated magnetic beads. Phage selections were performed for three rounds followed by screening by ELISA for Fab binding to hST2L-Fc protein. The selected Fab was affinity matured and converted to a human IgG1 format.
Application Notes
This antibody binds human ST2L and blocks the interaction between IL-33 and ST2L. This antibody was capable of recognizing recombinant human ST2L extracellular domain (ST2L-ECD) using an ELISA assay (PMID: 27294560). The original human IgG2 chimeric version of the antibody binds human and cynomolgus monkey ST2L with a binding affinity of 11 pM and 5 pM respectively (US9090694). Ths antibody inhibited IL-33-induced GM-CSF , IL-5, IL-8, and IL-13 release in primary human lung mast cells at antibody concentrations 100 μg/ml, 10 μg/ml and 1 μg/ml. Similar results were obtained using the cord blood-derived mast cells (US9090694). This antibody can be used for detection of ST2L using flow cytometry.
Antibody First Published
Fursov et al. Monoclonal antibodies targeting ST2L Domain 1 or Domain 3 differentially modulate IL-33-induced cytokine release by human mast cell and basophilic cell lines. Mol Immunol. 2016 Jul:75:178-87. PMID:27294560
Note on publication
Describes the generation and characterization of antibodies against Domain I and III of human ST2L.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
