Cat. No.
MABL-3384
Application
crystallization, functional assay, SPR, Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Cat (Felis catus)
Clone No.
AFD-REGN1909
From
Recombinant Antibody
Specificity
This antibody binds chain 2 of the Fel d 1, major cat allergen.
Alternative Names
Major cat allergen; CH2; AG4; Major allergen I polypeptide chain 2; Allergen Cat-1; Allergen Fel d I-B; Allergen FdI; Fel dI; Fel d 1-B
UniProt
P30440
Immunogen
The original antibody was generated by immunizing a Regeneron’s VelocImmune human antibody producing transgenic mouse with recombinant dimeric Fel d 1.
Application Notes
The original human IgG4 antibody was found to block binding of wild type Fel d 1 binding to IgE with a maximum blockade of 51% in a blocking ELISA. Surface plasmon resonance was used to identify the affinity of the antibody towards recombinant and wild type Fel d 1. Crystal structure of the Fab fragment antibody was studied using X-ray crystallography. This antibody in combination with REGN1908 was found to be promising in treatment of cat allergies. A single SC dose of REGN1908–1909 in patients with cat allergy resulted in rapid and clinically meaningful reduction in total nasal symptoms by blocking the early allergic response to nasal challenge with cat allergen (PMID: 29650949).
Antibody First Published
Orengo et al. Treating cat allergy with monoclonal IgG antibodies that bind allergen and prevent IgE engagement. Nat Commun. 2018; 9: 1421. PMID:29650949
Note on publication
Describes the generation of this antibody from a combinatorial IgE library derived from a doner and determines the clonal similarities between the antibodies generated.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
