Cat. No.
MABL-3328
Application
crystallography, ELISA
Isotype
Engineer antibody
Species Reactivity
German Cockroach (B. germanica)
Clone No.
AFD-7C11
From
Recombinant Antibody
Specificity
This antibody binds to the N-terminal lobe of Bla g 2.
Alternative Names
Cockroach Allergen; Aspartic protease Bla g 2; Blattella germanica allergen
UniProt
P54958
Immunogen
Bla g 2, a major cockroach allergen.
Application Notes
This antibody binds to Bla g 2 via an epitope commonly used by allergogenic IgE, however distinct from the epitope used by anti-Bla g 2 antibody clone 4C3 also available through Absolute Antibody. Both antibodies may be used in a sandwich ELISA. F(ab')2 versions of this antibody have been used in co-crystallization studies of Bla g 2.
Antibody First Published
Li et al. Crystal structure of a dimerized cockroach allergen Bla g 2 complexed with a monoclonal antibody. J Biol Chem. 2008 Aug 15;283(33):22806-14. PMID:18519566
Note on publication
Describes isolation of B-cells from patients, preparation of cDNA libraries as well as generation and characterization of anti-Phl p 5 scFv 4.2 .
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
