Cat. No.
MABL-3326
Application
Block, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AFD-TES-C21
From
Recombinant Antibody
Specificity
Alternative Names
Immunoglobulin E
UniProt
Immunogen
Polyclonal human IgE.
Application Notes
The antibody binds specifically to IgE, an immunoglobulin isotype secreted by upon stimulation of B cells by interleukins Il-4 and IL-13. IgE provides immunity to parasites such as helminths and Plasmodium falciparum. The immunoglobulin also plays a central role in the development of Type I hypersensitivity reactions and allergic diseases such as asthma, sinusitis and food allergies. These allergic responses are triggered by the binding of IgE to the high affinity receptor FcεRI, expressed on the surface of basophils and mast cells. Binding of IgE causes cross-linking of the receptor, inducing degranulation and release of inflammatory mediators, particularly of histamine. The monoclonal antibody TES-C21 binds to secreted IgE and to IgE expressed on the surface of IgE-producing B cells, thereby inhibiting IgE binding to FcεRI and the release of histamine from mast cells. The monoclonal antibody can have diagnostic applications, such as for identifying and quantifying IgE-expressing B cells and IgE levels in serum samples, and therapeutic applications in the treatment of allergies. Furthermore, the antibody can conjugated to cytotoxic drugs and deliver them specifically to IgE-producing B cells.
Antibody First Published
Kolbinger F, Saldanha J, Hardman N, Bendig MM. Humanization of a mouse anti-human IgE antibody: a potential therapeutic for IgE-mediated allergies. Protein Eng. 1993 Nov;6(8):971- 80. PMID:8309946
Note on publication
Describes the generation and characterisation of two monoclonal antibodies with cross reactivity to Der p 1 and Der f 1.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
