Cat. No.
MAK-0212
Product Name
Tn-C (multiple species)
Storage
4°C
Species
multiple species
Instructions
This ELISA specifically recognizes the fast-twitch skeletal muscle TnC isoform (F-TnC).
Introduction
The troponin complex regulates contraction of striated muscle. It is a heterotrimer of three polypeptides: troponin-I, troponin-C, and troponin-T. Two troponin-C (TnC) isoforms are expressed; one in fast-twitch skeletal muscle and one in both cardiac and slow-twitch skeletal muscle. This ELISA specifically recognizes the fast-twitch skeletal muscle TnC isoform (F-TnC). It can be used to investigate skeletal muscle injury. Antibodies used in the kit were generated against rabbit F-TnC.
PRINCIPLE OF THE ASSAY
F-TnC antibody is used for capture, immobilized on the microtiter wells and an HRP conjugated F-TnC antibody is used for detection. Standards and diluted serum samples (100 ul/well) are incubated in the microtiter wells for 45 minutes. The wells are then washed. HRP conjugate (100 ul/well) is added to the wells and incubated for 45 minutes. F-TnC molecules, if present, are sandwiched between the capture and detection antibodies. After washing the wells to remove unbound HRP-conjugate, TMB (100 ul/well) is added and incubated for 20 minutes. If F-TnC is present a blue color develops. Color development is stopped by addition of stop solution (100 ul/well), changing the color to yellow. Absorbance at 450 nm is then measured. The concentration of F-TnC is proportional to the absorbance and is derived from a standard curve.
