Anti-SARS-CoV-2 and SARS-CoV nucleocapsid mabs [mBG17](MABL-3592)

Cat. No.

MABL-3592

Name

Anti-SARS-CoV-2 and SARS-CoV nucleocapsid mabs 

Clone No.

mBG17

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF

Species Reactivity

SARS-CoV, SARS-CoV-2

Basic Information

Specificity

mBG17 strongly recognizes SARS-CoV-2 N  protein and SARS-CoV N protein. To lesser extent, it cross-reacts with the HuNL63 N protein. Western blot analysis with truncated fragments of N protein   suggested that its epitope resides in AA381-419 at the C-terminal.

Alternative Name

NP; nucleoprotein; NC; Protein N;   Nucleocapsid protein; SARS-CoV-2 Protein N; SARS-CoV-2 Nucleocapsid protein;   SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 17; SARS-CoV  nucleocapsid

UniProt

P0DTC9

Immunogen

The original mouse IgG1 version of this antibody was raised by immunizing BALB/c mice with a recombinant SARS-Cov-2 N protein corresponding to AA133-419.

Application Notes

Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2 and SARS- Cov was confirmed by   Specificty of the original mouse IgG1 version of this antibody to SARS-CoV-2   and SARS-Cov was confirmed by ELISA (Terry et al., 2021; pmid: 33714753).   This antibody detected SARS-Cov-2 N protein in Western blot exhibiting   labeling patterns suggesting a linear epitope (Terry et al., 2021; pmid:   33714753). mBG17 was shown to be very effective in staining SARS-Cov-2 N   protein in immunofluorescence in SARS-Cov-2-infected Vero cells, both   methanol and paraformaldehyde fixed (Terry et al., 2021; pmid: 33714753).   Finally, the original version of mBG17 was demonstrated to be an effective   detection antibody in a sandwich ELISA assay suggesting it's potential use in   SARS-Cov-2 diagnostics (Terry et al., 2021; pmid: 33714753).

Antibody First   Published

Terry et al. Development of SARS-CoV-2   Nucleocapsid Specific Monoclonal Antibodies Terry JS, Anderson LB, Scherman   MS, McAlister CE, Perera R, Schountz T, Geiss BJ. Development of a SARS-CoV-2   nucleocapsid specific monoclonal antibody. Virology. 2021 Jun;558:28-37.   PMID:33714753

Note on publication

Describes the generation and characterization of novel anti-SARS-CoV-2 N protein antibodies.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.