Cat. No.
MABL-3600
Application
biolayerinterferometry, FACS, NTRL, therapeutic, ELISA
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus2 (SARS-Cov-2)
Clone No.
MR17
From
Recombinant Antibody
Specificity
This antibody binds the receptor binding domain (RBD) of the SARS-CoV-2.
Alternative Names
SARS CoV 2 S glycoprotein; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
UniProt
P0DTC2
Immunogen
The original antibody was selected after one round of ribosome display using three high diversity libraries (concave, loop and covex) followed by three rounds of phage display and panning against biotinylated RBD as a bait and increasing the stringency of selection with every round. The last round of selection was against 5nM RBD.
Application Notes
Wild type MR17 had a modest neutralization activity. ELISA was used to screen the libraries to identify specific RBD binders. Neutralization activity of the sybody was checked by pre- incubating pseudoviral particles with different concentrations of sybody before infection of VeroE6-hACE2 cells. The rate of infection was then measured by fluorescence-activated cell sorting (FACS). IC50 value of wild type MR17 was determined to be 12.32 µg m/L. This sybody is a K99Y mutant version of wild typr MR17. It is seen that the single mutation increased the binding affinity by 1.5 fold and decreased the off- rate by 2.8 fold. Overall neutralization activity was increased 24 fold after the mutation was introduced, with an IC50 value of 0.50 µg m/L. The binding kinetics between sybodies and the RBD was assessed using bio-layer interferometry (Li et al., 2020).
Antibody First Published
Li et al. Potent synthetic nanobodies against SARS-CoV-2 and molecular basis for neutralization. BioRxiv (2020)
Note on publication
Describes the generation, neutralization activity, structural analysis and mutant version of the sybody with superior binding and neutralizing activity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
