Cat. No.
MABL-3577
Application
NTRL, ELISA
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus
Clone No.
1B5
From
Recombinant Antibody
Specificity
This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein (Urbani strain).
Alternative Names
Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1
UniProt
P59594
Immunogen
The original antibody was generated by immunizing a non human transgenic animal XENOMOUSE® IgG2k mice against S1 protein-Ig fragments (ectodomain) of the S Protein (Tor2).
Application Notes
This antibody inhibits the association of SARS-CoV s protein to the angiotensin- converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the virus. The originally characterized antibody had a neutralizing titer 200TCID50 of 12.5 μg/ml (Coughlin et at.,2006) and IC50 of 2.84 μg/ml when incubated with S12–510- Fc recombinant protein (Coughlin et at.,2009). Purified 1B5 binds to RBD region with a unique CDR3 sequence. When used in combination with 4D4 the lack of synergistic effect indicated that the mAbs 4D4 and 1B5 likely interact with closely positioned conformational epitopes and compete with each other for binding (Coughlin et at.,2009). ELISA.
Antibody First Published
Coughlin et al. Generation and characterization of human monoclonal neutralizing antibodies with distinct binding and sequence features against SARS coronavirus using XenoMouse® Virology 361 (2007) 93–102. PMID:17161858
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
