Cat. No.
MABL-3575
Application
NTRL, ELISA
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus
Clone No.
6B1
From
Recombinant Antibody
Specificity
This antibody specifically binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein (Urbani strain).
Alternative Names
Spike protein; S protein; SARS-CoV S protein; S glycoprotein; E2; Peplomer protein; Spike protein S1
UniProt
P59594
Immunogen
The original antibody was generated by immunizing a non human transgenic animal XENOMOUSE® IgG2k mice against S1 protein-Ig fragments (ectodomain) of the S Protein (Tor2).
Application Notes
This antibody inhibits the association of SARS-CoV s protein to the angiotensin- converting enzyme 2 (ACE2) receptor by binding to specific amino acids on the S1 domain of the spike protein of the virus. The originally characterized antibody had has a neutralizing titer 200TCID50 of 3.125 μg/ml (Coughlin et at.,2006) and IC50 of 4.08μg/ml when incubated with S12–510- Fc recombinant protein (Coughlin et at.,2009). This antibody 6B1 showed about 50% binding to Sin845 S1 protein relative to their binding to Urbani S1 protein. GZ0402-S1 protein showed minimal of 52% binding to HmAbs 6B1 (Elshabrawy et al., 2012). Other applications include ELISA.
Antibody First Published
Coughlin et al. Generation and characterization of human monoclonal neutralizing antibodies with distinct binding and sequence features against SARS coronavirus using XenoMouse® Virology 361 (2007) 93–102. PMID:17161858
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
