Key features and details | |
Cat. No. | MABL-3431 |
Name | Anti-EE-tag mAbs [3D5/EE_48.K](MABL-3432) |
Clone No. | 3D5/EE_48.K |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | EE tag |
Basic Information | |
Specificity | This antibody recognizes and binds a six amino acid long sequence (EYMPME). |
Alternative Name | EE epitope tag (EYMPME); Glu-Glu epitope tag |
UniProt | |
Immunogen | This scFv antibody was generated by previously crystallized hexa-histidine-specific 3D5 scFv antibody. The 3D5 scFv was modified in the complementary determining region by random mutagenesis, in conjunction with phage display, to yield scFvs with new biochemical characteristics and binding specificity. |
Application Notes | This antibody successfully identifies protein with either internal or C-terminal EE peptides in western blot assays. When expressed in soluble form they recognize the EE peptides in ELISA (PMID:21217145; PMID:24615866). |
Antibody First Published | Pai et al. Conversion of scFv peptide-binding specificity for crystal chaperone development. Protein Eng Des Sel. (2011); 24(5): 419–428. PMID:21217145 |
Note on publication | Describes the generation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
