Anti-Protein C mAbs [HPC-4](MABL-3429)

Cat. No.

MABL-3429

Name

Anti-Protein C mAbs [HPC-4](MABL-3429)

Clone No.

HPC-4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, Purification, ELISA

Species Reactivity

Baboons, Human

Basic Information

Specificity

Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of   the Protein C (spanning the thrombin cleavage site). Does not react with   activated protein C. Has been shown to be cross-reactive with baboon Protein   C but not with bovine or mouse Protein C.

Alternative Name

Autoprothrombin IIA; C-tag; Protein C tag; Protein C-tag; HPC4; Vitamin K-dependent protein C; Anticoagulant protein C; Blood coagulation factor XIV; PROC

UniProt

P04070

Immunogen

This antibody was raised by immunising mice with human protein C.

Application Notes

HPC-4 binds human   protein C only in the presence of Ca2+; it binds to the reduced   carboxymethylated protein C heavy chain, and it blocks protein C activation   but does not bind or inactivate protein C or activated protein C bound to its   plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it   recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987; 3102560). It   has been shown to work in ELISAs to determine expression levels (Velander et   al., 1992; PMID: 1465430). HPC-4 has been shown to be effective at purifying   proteins tagged with its epitope (Rezaie et al., 1992; PMID: 1283093). It has   also been used to block the activation of Protein C by the   thrombin-thrombomodulin complex (Stearns et al., 1988; PMID: 2447082) and was   used for the same purpose in Baboons to lower the threshold of sepsis (Taylor   Jr et al., 1987; PMID: 3102560). HPC-4 has been used in many other studies to   observe the function of Protein C by knocking out its function in a   Ca2+-dependent manner, purification, and others (Tanaka et al., 2006; PMID:   16398653) (Bae et al., 2007; PMID: 17823308).

Antibody First   Published

Taylor et al. Protein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in   the baboon. J Clin Invest. 1987 Mar;79(3):918-25. PMID:3102560

Note on publication

Describes the generation and characterisation of the HPC-4 antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.