Anti-Rhodopsin mAbs [Rho 1D4](MABL-3419)

Cat. No.

MABL-3419

Name

Anti-Rhodopsin mAbs [Rho 1D4](MABL-3419)

Clone No.

Rho 1D4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, Radioimmune labeling, WB, ELISA, IF, IHC  

Species Reactivity

Cow, Human, Zebrafish, Amphibians, Rat, Mouse

Basic Information

Specificity

The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The   epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of   the 1D4 tag, however, is that it has to be placed at the C-terminus of a   protein. This is because the Rho1D4 monoclonal antibody requires a free   carboxylate group for high affinity binding. Amidation of the carboxyl group   lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold.

Alternative Name

Rho-tag; Visual Purple

UniProt

P08100

Immunogen

Bleached bovine rod outer segment (ROS).

Application Notes

The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes.   Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein- coupled receptors (GPCRs). The linear epitope   (TETSQVAPA) can be used as a protein tag.

Antibody First   Published

Molday et al. 1983 Monoclonal antibodies to rhodopsin: characterization, corss-reactivity, and applicatoin as sturctural probes. Biochemistry, 1983; 22(3):653-660 PMID:6188482

Note on publication

Describes the generation of two monoclonal antibodies from hybridoma.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.