Key features and details | |
Cat. No. | MABL-3419 |
Name | Anti-Rhodopsin mAbs [Rho 1D4](MABL-3419) |
Clone No. | Rho 1D4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, Radioimmune labeling, WB, ELISA, IF, IHC |
Species Reactivity | Cow, Human, Zebrafish, Amphibians, Rat, Mouse |
Basic Information | |
Specificity | The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. The epitope has the following amino acid sequence: 'TETSQVAPA'. A limitation of the 1D4 tag, however, is that it has to be placed at the C-terminus of a protein. This is because the Rho1D4 monoclonal antibody requires a free carboxylate group for high affinity binding. Amidation of the carboxyl group lowers its immunoreactivity to the Rho1D4 antibody by over 100 fold. |
Alternative Name | Rho-tag; Visual Purple |
UniProt | P08100 |
Immunogen | Bleached bovine rod outer segment (ROS). |
Application Notes | The antibody binds to the C-terminus of rhodopsin on the cytoplasmic side of disk membranes. Rhodopsin is the major pigment in rod photoreceptors and belongs to the family of G-protein- coupled receptors (GPCRs). The linear epitope (TETSQVAPA) can be used as a protein tag. |
Antibody First Published | Molday et al. 1983 Monoclonal antibodies to rhodopsin: characterization, corss-reactivity, and applicatoin as sturctural probes. Biochemistry, 1983; 22(3):653-660 PMID:6188482 |
Note on publication | Describes the generation of two monoclonal antibodies from hybridoma. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
