Anti-His tag (C-term) mAbs [3D5](MABL-3416)

Cat. No.

MABL-3416

Name

Anti-His tag (C-term) mAbs [3D5](MABL-3416)

Clone No.

3D5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FACS, WB, ELISA, FC Published Species Reactivity

Species Reactivity

Species independant

Basic Information

Specificity

This antibody binds to carboxy-terminal   oligo-histidine tags (6x His tags) of fusion proteins. It also has affinity   for internal His-tags but no affinity for N-terminal His-tags.

Alternative Name

polyhistidine tag

UniProt

Immunogen

Purified his tagged Fab fragment.

Application Notes

This antibody was   successfully used for ELISA, WB, and FACS. Monomeric and dimeric scFv   versions of the antibody were generated and described (Lindner et al., 1997;   PMID: 8994661). The scFv version of this antibody was engineered into a lentiviral vector system to confer specificity for cells displaying the His   tag on their surface, enabling targeted gene transfer into HT1080-anti-His   cells (Friedel et al., 2015; PMID: 25715658). This antibody was used in FC to   detect Canine IL-17A in Canine IL-17A- transfected HEK293 A cells (Akiyama et   al., 2019; PMID: 30885301).

Antibody First   Published

Lindner P, Bauer K,   Krebber A, Nieba L, Kremmer E, Krebber C, Honegger A, Klinger B, Mocikat R,   Plückthun A. Specific detection of his-tagged proteins with recombinant   anti-His tag scFv-phosphatase or scFv-phage fusions. Biotechniques. 1997 Jan;22(1):140-9. PMID:8994661

Note on publication

Describes the making of the antibody, shows it recognised carboxy-terminal oligo- histidine tags (His   tags) of a wide variety of proteins in immuno-assays of cell lines with His tag amplifications.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.