Cat. No.
MABL-3968
Application
ELISA, IHC, Imaging
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HMFG2
From
Recombinant Antibody
Specificity
This antibody recognises MUC1, a tumor associated antigen which is expressed in >90% ovarian carcinomas. This antigen is a high molecular weight (M, 80,000-200,000) glycoprotein.
Alternative Names
Muc-1
UniProt
P15941
Immunogen
Human breast epithelial mucin.
Application Notes
In an attempt to develop a reliable way to detect breast cancer, immunoprecipitation was preformed on NPGP from HMFG using the mouse version of this antibody bound to Sepharose 4B. Furthermore, this antibody was tested in an radioimunnology experiment on NPGP using the mouse version of this antibody (Ceriani et al, 1992; pmid:1377884). The epitope of this antibody was mapped by ELISA using small peptides - the murine version of this antibody was used (Petrakou et al, 1998 ; pmid:9422085). This antibody was used to identify cells expressing the MUC1 protein. This was accomplished by using the mouse version of this antibody on cell lysates of cell transfected with the MUC1 protein in a western blot analysis (Rubinstein et al, 2006; pmid:17145869). To compare the diagnostic usefulness of different antibodies, the mouse version of this antibody was used for immunohistochemistry on cell pellets from pleural fluid specimens (Creaney et al, 2008; pmid:18454162). While developing an antibody against the alpha/beta junction of MUC1 the mouse version of this antibody was used for western blot on the MUC1 protein (Rubinstein et al, 2008; pmid:18821582).
Antibody First Published
Christian et al. 1996 Cloning and expression of cDNAs encoding the variable domains of the anti-breast carcinoma antibody Mc5. Hybridoma 1996; 15(2):155-158 PMID:8743296
Note on publication
Describes how Mc5 has been used as a reagent in the diagnosis of breast cancer.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
