Cat. No.
MABL-3965
Application
IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
41-28
From
Recombinant Antibody
Specificity
This antibody binds the human megakaryocyte-potentiating factor.
Alternative Names
Megakaryocyte-potentiating factor; MSLN; CAK1 antigen; mesothelin
UniProt
Q13421
Immunogen
This antibody was raised by immunising Balb/c mice with EMA prepared from human milk fat globule.
Application Notes
E29 antibody has been widely used in studies on various types of neoplastic conditions and is recommended for immunohistochemistry alone or with other antibodies for formalin-fixed, paraffin- embedded tissue characterisation. For instance, Tajima and Koda (2015) used it as a part of immunohistochemical characterisation of the ectomesenchymal chondromyxoid tumor of the tongue. Another group immunohistochemically stained formalin-fixed pancreatic tumour tissue with E29 in order to label EMA (MUC1) (Woei-A-Jin et al., 2016). EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms, e.g. Schwannomas. Finally, the absence of EMA staining may also be used as an important factor in tissue characterisation and cancer diagnosis. Lack of EMA signal is typical for certain tumours, such as adrenal carcinoma, seminomas, paraganglioma and hepatoma.
Antibody First Published
Cordell et al. Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Br J Cancer. 1985 Sep;52(3):347-54. PMID:3899155
Note on publication
This article describes generaton and characterisation of E29 antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
