Cat. No.
MABL-3934
Application
ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
4D5-8 (trastuzumab)
From
Recombinant Antibody
Specificity
Oncogenic protein Her-2/neu (erbB-2), a member of the family of epidermal growth factor (EGF) receptors, overexpressed in 30% of invasive breast cancers and 70% of ductal carcinomata in situ, in a small percentage of melanomas, and in other malignancies originating from various organs, including ovary, kidney, colon, and bladder.
Alternative Names
CD340; Metastatic lymph node gene 19 protein (MLN 19); Proto- oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; p185HER2; EGFR
UniProt
P04626
Immunogen
Peptide "GLQSLPTHDPSPLQR" derived from the receptor tyrosine kinase erbB2 conjugated to KLH.
Application Notes
Original data characterizing this antibody may be found here .
Antibody First Published
Schoenherr et al. Anti-peptide monoclonal antibodies generated for immuno-multiple reaction monitoring-mass spectrometry assays have a high probability of supporting Western blot and ELISA. Mol Cell Proteomics PMID:25512614
Note on publication
Describes generation a panel of recombinant antibodies using synthetic peptides of human proteins as well as validation using ELISA and multiple reaction monitoring-mass spectrometry.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
