Cat. No.
MABL-3932
Application
tumor imaging, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
scFv78
From
Recombinant Antibody
Specificity
This antibody binds a conformational epitope on the human and mouse endosialin. Endosialin (also known as tumor endothelial cell marker molecule 1, TEM1/CD248) is a type I transmembrane protein, composed of a glycosylation modified 80.9KDa protein core region, the modified mature glycoprotein is about It is 175KDa. TEM1 is one of the human tumor markers, which plays an important role in tumor cell growth, angiogenesis, invasion and metastasis. Tumor endothelial cell marker molecule 1 (TEM1/CD248) is identified as a microvascular marker of tumor angiogenesis in various human cancers and highly expressed on a variety of solid tumors (endothelial cells and stromal cells), such as sarcoma, brain tumor, breast cancer, skin cancer, colon cancer, and experiments have shown that in ovarian cancer, The expression of TEM1 is closely related to tumor invasion and prognosis.
Alternative Names
CD248; TEM1; CD164L1; FB5; tumor endothelial marker 1; Biobody-78
UniProt
Q9HCU0
Immunogen
Mice were immunized with membrane-enriched fractions of human metastic mammary carcinoma cells. The spleens were extracted and fused with NS-1 to generate primary hybridoma cultures. These were screened using solid phase RIA for those which were reactive with metastic mammary tumor cells from livers but were not reactive with healthy livers.
Application Notes
Has been used to generate a human engineered monoclonal antibody (Ammons et al, 2003). B38.1 has been shown to be able to mediate antibody-dependent cytotoxicity (Robinson et al, 1991). It has undergone clinical trials as an anti-carcinoma drug (de Bono et al, 2002 and Better et al, 2002). It has also been used in ELISAs to test the reactivity of its humanised construct, ING-1, that it has an affinity in the nM range by competition assays, and it has been shown to be active in vivo (Ammons et al, 2003).
Antibody First Published
Colcher et al Monoclonal antibodies reactive with human breast cancer Proc Natl Acad Sci U S A. 1981 May;78(5):3199-203 PMID:6789331
Note on publication
Describes the formation of this antibody and others against metastic mammary carcinoma cells. Describes the characterisation of these antibodies including identifying a number of carcinoma types, other than mammary, that they are reactive against.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
