Cat. No.
MABL-3911
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
EB-022
From
Recombinant Antibody
Specificity
EB-022 was shown by ELISA to react specifically with CEA (CD66e), as opposed to the related CD66 family member CD66c (also known as NCA; unpublished data).
Alternative Names
Carcinoembryonic antigen; Carcinoembryonic antigen-related cell adhesion molecule 5; CEACAM5; Cluster of Differentiation 66e; CD66e
UniProt
P06731
Immunogen
The original antibody was generated by immunizing female BALB/c mice subcutaneously with soluble CEA or with the HC84S cell line.
Application Notes
The binding affinity of the original mouse antibody to carcinoembryonic antigen was determined using a competitive radioimmunoassay (PMID: 6187848). 111In (Indacea) labelled version of this antibody was used to image tumors in patients with colorectal cancer (PMID: 3779658). An engineered antibody fragment (VL-VH-CH3, or "minibody") generated using this antibody having bivalent binding to carcinoembryonic antigen showed high level targeting of xenografts (PMID: 8674062). A radiolabeled chimeric version of this antibody was used to target breast cancer cells expressing high levels of CEA and it was found that chimeric 86.66 resulted in good tumor localization as well as significant tumor growth inhibition. This antibody was also used to detect CEA expression in Human breast carcinoma cell line, MCF 7, using flow cytometry and immunohistochemistry (PMID: 21597719). The characterization of the chimeric version of this antibody generated by transient expression in tobacco leaves was done using ELISA (PMID: 10500141).
Antibody First Published
Wagener et al. Monoclonal antibodies for carcinoembryonic antigen and related antigens as a model system: determination of affinities and specificities of monoclonal antibodies by using biotin-labeled antibodies and avidin as precipitating agent in a solution phase immunoassay. J Immunol. 1983 May;130(5):2302-7. PMID:6187848
Note on publication
Describes the use of competitive radioimmunoassay as a method for determination of affinity constants and antigen cross-reactivities of monoclonal antibodies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
