Rabbit anti-SP-1 Chromogranin A (Porcine) Antibody

Name

Rabbit anti-SP-1 Chromogranin A (Porcine) Antibody

中文名字

兔抗SP-1嗜铬粒蛋白A（猪）抗体

Description

The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/500-1/1000 dilution, in rat adrenal medulla and rat stomach.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Mouse, Rat

Preabsorption Control

/

Alternate Names

Pituitary secretory protein I; CgA; chromogranin A (parathyroid secretory protein 1), anti-SP-1 Chromogranin A

RRID

AB_572226

Immunogen

This information is proprietary to Mabioway

Gene Symbol

CHGA

Entrez Gene ID

Entrez Gene: 102413232   Buffalo
Entrez Gene: 423420        Chicken
Entrez Gene: 281070        Cow
Entrez Gene: 101318300   Dolphin
Entrez Gene: 1113            Human
Entrez Gene: 397540        Mouse
Entrez Gene: 403993        Pig
Entrez Gene: 101114907   Ram (Sheep)
Entrez Gene: 24258          Rat

NCBI Gene Aliases

/

Sequence

403993

APPLICATION

Quality Control

The antibody produces significant immunofluorescent staining and significant Biotin/avidin-HRP staining at a 1/500–1/1,000 dilution.

Tissue

Rat adrenal medulla and stomach (antrum)

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20-30 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjugation with paraformaldehyde.

Absorption Control

Sections

10 µm cryostat

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn-AV/HRP reagents at dilutions recommended by the manufacturers.

Suggested Dilution

1/500–1/1,000 in PBS/0.3% Triton X-100–Bn-AV/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles