Rabbit anti-GluR1 (Ionotropic Glutamate Receptor 1) Antibody

Name

Rabbit anti-GluR1 (Ionotropic Glutamate Receptor 1) Antibody

中文名字

兔抗GluR1抗体;兔抗谷氨酸受体1抗体

Description

The antibody produces strong labeling of GluR1 at dilutions of 1/4,000 – 1/6,000 using biotin-streptavidin peroxidase technique in rat cortex and hippocampus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Western blot analysis of GluR1 transfected cells and rat brain homogenates the antibody specifically labels a single band at approximately 102 kD. Western blot analysis of GluR2, 3, 4, 4C, 5, 6, and 7 transfected cells revealed no immunolabeling. Immunolabeling of the above non-NMDA transfected cells demonstrates specificity for GluR1. Additionally, immunolabeling for GluR1 is completely abolished by pre-adsorption with synthetic rat GluR1 (894-907) at 5 µg per mL of diluted antibody.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

 AMPA-selective glutamate receptor 1; AMPA1; GLUH1; GluR-A; GluR-K1; Glutamate receptor ionotropic,AMPA1; Glutamate receptor ionotropic, kainate 1; GluK1; GluA1; glutamate receptor, ionotropic, AMPA1 (alpha 1), anti-GluR1

RRID

AB_572243

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Gria1

Entrez Gene ID

NCBI Gene Aliases

/

Sequence

P42261

APPLICATION

Quality Control

The antibody produces significant labeling of GluR1 at dilutions of 1/4,000–1/8,000 using biotin-avidin peroxidase technique in rat cortex and hippocampus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. Using western blot analysis of GluR1 transfected cells and rat brain homogenates the antibody specifically labels a single band at approximately 102 kD. Western blot analysis of GluR2, 3, 4, 4C, 5, 6, and 7 transfected cells revealed no immunolabeling. Immunolabeling of the above non-NMDA transfected cells demonstrates specificity for GluR1. Additionally, immunolabeling for GluR1 is completely abolished by preadsorption with synthetic rat GluR1 (894–907) at 5 mg per mL of diluted antibody

Tissue

Rat cortex and hippocampus

Perfusion Fixation

• Fixative - 4% paraformaldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post fixation - 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4. .
• Note: If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Bn/AV-HRP reagents at dilutions recommended by the manufacturer.

Suggested Dilution

1/4,000–1/8,000 in PBS/0.3% Triton X-100 - Bn/AV-HRP technique

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long term storage, aliquot and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles.