Key features and details | |
Cat. No. | MABL-2350 |
Name | Anti-MET mAbs |
Clone No. | AFD- 7A2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibition, SPR, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody specifically binds to amino acid residues 568-741 of human MET. |
Alternative Name | human HGF receptor; 084-G02; Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met |
UniProt | P08581 |
Immunogen | The original antibody was selected by biopanning with a scFv phage library performed on solid-phase recombinant MET928. |
Application Notes | The scFv fragment showed inhibition of HGF/SF induced migration of SKOV cells. In particular, the scFv fragment had an IC50 of 2.3 nM in the SKOV migration assay. The binding affinity of the scFv fragment to MET928 was measured by surface plasmon resonance (Kd= 3.6 nM). In a competition ELISA, binding of 2.5 nM of the Fab version of the antibody to a MET928-coated surface was inhibited by 15 μM and 5 μM NK1 (US20150147274A1). |
Antibody First Published | DiCara et al. Characterization and structural determination of a new anti- MET function-blocking antibody with binding epitope distinct from the ligand binding domain Sci Rep. 2017 Aug 21;7(1):9000 |
Note on publication | The original paper describes the generation and characterization of the antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
