Key features and details | |
Cat. No. | MABL-1441 |
Name | Anti-DUX4 mAbs |
Clone No. | AFD- P2B1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody recognises C-terminal fragment of human DUX4-fl, aa 349-424. It does not cross- react with DUX4c. DUX4 is a protein thought to be engaged in facioscapulohumeral dystrophy (FSHD), one of the most common muscular dystrophies. |
Alternative Name | Double homeobox protein 4; Double homeobox protein 10; DUX10; DUX4L1; P2B1-B3 |
UniProt | Q9UBX2 |
Immunogen | This antibody was raised by immunising mice with C-terminal fragment of human of DUX4 (aa 349-424) with glutathione-s-transferase (gst) tag. |
Application Notes | P2B1 antibody can be used to assess the protein expression of DUX4 in muscle cells affected by facioscapulohumeral dystrophy (FSHD). For instance, this antibody was used to stain C2C12 myoblasts transfected with human DUX4 (Geng et al., 2011). |
Antibody First Published | Geng et al. Immunodetection of human double homeobox 4. Hybridoma (Larchmt). 2011 Apr;30(2):125-30. doi: 10.1089/hyb.2010.0094. PMID:21529284 |
Note on publication | This article describes the generation and characterisation of the P2B1 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
