Anti-DR4 mAbs

Cat. No.

MABL-1430

Name

Anti-DR4 mAbs

Clone No.

AFD- mAY4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, WB, ELISA

Species Reactivity

Human

Basic Information

Specificity

mAY4 was shown to bind DR4 by ELISA (Sung et   al, 2009). These experiments also showed that mAY4 did not react with other   homologous death receptors, including DR5, decoy receptor (DcR) 1, DcR2, FAS   receptor, and mTR (a mouse ortholog of human DR4 and DR5). SPR showed an   affinity of 7.1 nM for interaction with DR4. mAY4 bound to a different epitope on DR4 than the physiological ligand TRAIL. DR4 is a pro-apoptotic   receptor expressed on all cells. It represents a promising target for   anti-cancer therapeutic agents.

Alternative Name

CD261; Death receptor 4; Tumor necrosis   factor receptor superfamily member 10A; TNF-related apoptosis-inducing ligand   receptor 1; TRAIL receptor 1; TRAILR1; TRAIL-R1; TRAILR 1; TNFRSF10A; APO2

UniProt

O00220

Immunogen

mAb mAY4 was generated by immunising BALB/c   mice with the ECD (residues 1-215) of human DR4. Splenocytes were obtained   from immunised mice and fused with murine Sp2/O myeloma cells. mAY4 was   cloned by RT-PCR and reformatted as an scFv.

Application Notes

mAY4 was shown to bind DR4, but not other   related homologs, using ELISAs and SPR (Sung et al, 2009). The mAb and  scFv-reformatted version were also used in cell viability assays for   assessment of tumouricidal activity. More recently, the mAY4 CDRs were   humanised and expressed as an scFv-reformatted antibody fragment (Lee et al,   2010), although the humanised version had a 10-fold lower affinity for DR4.

Antibody First Published

Sung et al. A novel agonistic antibody to   human death receptor 4 induces apoptotic cell death in various tumor cells   without cytotoxicity in hepatocytes. Mol Cancer Ther. 2009 Aug;8(8):2276-85.   PMID:19638452

Note on publication

Describes the generation of mAY4, a murine antibody against human DR4. The mAb, and a scFv-reformatted version,were shown to promote DR4-mediated apoptotic activity and prevent tumour growth of   human NSCLC tumours established in mice.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.