Anti-DOTA (metal-bound) mAbs

Cat. No.

MABL-1424

Name

Anti-DOTA (metal-bound) mAbs

Clone No.

AFD- 2D12.5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

radioimmunotherapy

Species Reactivity

Species independent

Basic Information

Specificity

This antibody binds metal bound DOTA   (Tetraxetan) and also shows binding affinity for Yttrium(III) labeled DOTA,   (S) nitro benzyl-DOTA chelates and Janus-DOTA (a bivalent form of DOTA). DOTA   is a chelating agent which can be labelled with radioactive yttrium ion and   even conjugated with monoclonal antibodies for radioimmunotherapy.

Alternative Name

Tetraxetan; dodecane tetra acetic acid; CHEBI:61028; Yttrium(III) labeled DOTA; Y-DOTA; Yttrium-1 4 7 10-tetraazacyclododecanetetraacetic acid; metal-DOTA

UniProt

Immunogen

The original antibody was generated by   immunizing BALB/c mice with KLH-21T-BAD-Y(III).

Application Notes

The original mouse IgG1 antibody binds DOTA chelates   of all the rare earths with approximately Kd ≈ 10(-8) M, making it useful for   the capture of probe molecules with a variety of properties (PMID: 15546206).   The original IgG1 version of this antibody was used in the pretargeting for   radioimmunotherapy in BALB/c mice with KHJJ tumors (PMID: 7954426). In vivo   clearance studies have demonstrated that 74-96% of 2D12.5 bound to metal   chelates is present 24 hours after administration of the bound complex   (US7528235). An engineered version of this antibody with a single cysteine   residue at position 54, 55 or 56 of the heavy chain, a site proximal to the   protein's binding site, enables formation of weakly electrophilic metal   complexes of (S)-2-(4-acrylamidobenzyl)-DOTA (AABD) that may bind and form   permanent linkages (PMID: 15546206; 15546207). 

Antibody First Published

Goodwin et al. Pharmacokinetics of   pretargeted monoclonal antibody 2D12.5 and 88Y-Janus-2-(p-nitro   benzyl)-1,4,7,10-tetraazacyclododecanetetraacetic acid (DOTA) in BALB/c mice   with KHJJ mouse adenocarcinoma: a model for 90Y radioimmunotherapy. Cancer   Res. 1994 Nov 15;54(22):5937-46. PMID: 7954426

Note on publication

Describes the pretargeting and pharmacokinetics of antibody 2D12.5 and DOTA for radioimmunotherapy in mouse models

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.