Key features and details | |
Cat. No. | MABL-1421 |
Name | Anti-domoic acid mAbs |
Clone No. | AFD- 2H12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for domoic acid. Domoic acid is a kainic acid-type neurotoxin that causes amnesic shellfish poisoning. It is produced by algae and accumulates in shellfish, sardines, and anchovies. When sea lions, otters, cetaceans, humans, and other predators eat contaminated animals, poisoning may result. |
Alternative Name | DA; (2S,3S,4S)-4-[(2Z,4E,6R)-6-Carboxyhepta-2,4-dien-2-yl]-3- (carboxymethyl)pyrrolidine-2-carboxylic acid |
UniProt | |
Immunogen | |
Application Notes | The specificity of the original format and the scFv format of the antibody was confirmed by ELISA analysis (IC50 = 1 μg/ml and 245 ng/ml respectively, and Kd = 0.8 μM for the scFv fragment) (Hu et al., 2005; PMID: 16019098). The scFv was employed for the development of a novel immuno-sensor for in situ domoic acid screening, where the fragment was immobilized on mesoporous silica. Domoic acid binding analysis found that the adsorbed scFv bound domoic acid in a concentration- dependent manner (Hu et al., 2007; PMID: 17005419). The scFv antibody fragment was engineered with cysteine-containing linkers of two different lengths. The domoic acid detection ability of the covalently immobilized antibody fragments was compared with that of scFvs adsorbed non-covalently onto polystyrene plates. |
Antibody First Published | Hu et al. Cloning, expression and characterisation of a single-chain Fv antibody fragment against domoic acid in Escherichia coli J Biotechnol. 2005 Oct 17;120(1):38-45. doi: 10.1016/j.jbiotec.2005.05.018. Epub 2005 Jul 12. PMID:16019098 |
Note on publication | The original paper describes the cloning, expression and characterization of a single chain Fv antibody from a mouse hybridoma cell line. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
