Anti-DNA/RNA G-quadruplex mAbs

Cat. No.

MABL-1415

Name

Anti-DNA/RNA G-quadruplex mAbs

Clone No.

AFD- BG4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

EMSA, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

Binds with high selectivity and low   nanomolar affinity to DNA and RNA G-quadruplex structures.

Alternative Name

G4 quadruplex

UniProt

n/a

Immunogen

DNA/RNA G-quadruplex.

Application Notes

This antibody has been shown (by ELISA) to   bind selectively to G-quadruplex forming DNA and RNA structures. Please that   Fab-based versions as well as scFvs, with and without Flag-tag are available,   which needs to be considered when replicating published methods. Tsai et al.   (2022 et al. PMID: 36440760), report similar activtiy between whole-antibody   and scFv-based versions of BG4. Weldon et al. (2016, PMID: 27820800) used   Ab00174-1.1 to identify G-quadruplexes in long functional RNAs using an   electrophoretic mobility shift assay (EMSA). The mouse IgG1 version of BG4   was shown to have weak cross- reactivity with iMotif structures (2018, PMID:   29686376). iMotif structures can be detected selectively using our   anti-iMotif antibody Ab01462.

Antibody First Published

Biffi G, Tannahill D, McCafferty J,   Balasubramanian S. Quantitative visualization of DNA G-quadruplex structures   in human cells. Nat Chem. 2013 Mar;5(3):182-6. PMID:23422559

Note on publication

Describes generation of a   G-quadruplex-specific antibody using phage display and the in vitro selection   (using ELISA and CD) of a structure-specific antibody probe that binds with   high selectivity and low nanomolar affinity to DNA G-quadruplex structures   called BG4.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.