Key features and details | |
Cat. No. | MABL-1392 |
Name | Anti-dioxin mAbs |
Clone No. | AFD- DD3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | EIA, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody recognizes tetrachloro- and pentachloro-dibenzodioxins and -dibenzofurans. It falls to bind either non-chlorinated, mono-, hexa-, or octa-chlorinated dibenzodioxins, nor does it recognize non-chlorinated, octachloro- or 1,2,3,4,8,9-hexachlorodibenzofurans. Dioxins are a group of chemical compounds that are persistent organic pollutants (POPs) in the environment. |
Alternative Name | PCDD; DLCs; PCDF; TCDD; DD-3 |
UniProt | |
Immunogen | The antibody was generated by immunizing BALB/c mice with 1-N-(adipamino)-2,3,7-triCDD- linked carrier-protein BSA. |
Application Notes | The specificity of the antibody for 2,3,7,8-TCDD was confirmed by ELISA analysis. In order to evaluate the ability of the antibody to recognize various kinds of polychlorinated dibenzodioxins and dibenzofurans, a competition ELISA was developed. The antibody was specific and it recognized only a limited number of dioxin and dibenzofuran isomers. The antibody is suitable for the development of rapid, inexpensive screening assays for monitoring the presence of dioxin in biological, soil and other environmental samples (Stanker et al., 1987; PMID: 3629609). Immunoaffinity columns were prepared using the antibody and employed for purification (Huwe et al., 2001; PMID: 11417873) or recovery (Shelver et al., 2002) of polychlorinated dibenzo-p-dioxins and furans from biological samples. The recombinant Fab version of the antibody was generated; it displayed competitive inhibition with 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) in an indirect ELISA (Lee et al.; 1998). |
Antibody First Published | Stanker et al. Monoclonal antibodies for dioxin: antibody characterization and assay development Toxicology. 1987 Sep;45(3):229-43. PMID:3629609 |
Note on publication | The paper describes the generation and characterization of a set of 5 anti-dioxin monoclonal antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
