Key features and details | |
Cat. No. | MABL-1386 |
Name | Anti-digoxin mAbs |
Clone No. | AFD- 1B3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, therapy |
Species Reactivity | Species independet |
Basic Information | |
Specificity | 1B3 antibody recognises digoxin and digitoxin with an excellent binding affinity of Kd = 3 and 0.6 nM, respectively. Digoxin and digitoxin are both cardiac glycosides used widely in cardiology to treat various heart conditions, such as heart failure. |
Alternative Name | 12β-hydroxydigitoxin; digitalis; 12-beta-hydroxydigitoxin |
UniProt | |
Immunogen | This antibody was raised using transgenic HC2/KCo5 mice with the humanised humoral immune system capable of generating human IgG1 κ Abs.The mice were immunised with digoxin conjugated to keyhole limpet hemocyanin (KLH). |
Application Notes | Digoxin is widely used in various heart conditions. However, the drug has a very narrow therapeutic index and can cause toxic side effects. The antibody might neutralise the activity of digoxin and could be used as an antidote in case of overdose of digitalis (Paula et al., 2005). It is additionally superior to the widely utilised Fab preparations of sheep polyclonal anti-digoxin Abs as it is a humanised antibody which will not activate the immune system in patients (Ball et al., 1999). Anti-digoxin monoclonal antibodies can also be used to measure serum levels of the drug as a guide to therapy, and are useful in the investigation of the kinetics of interaction between antibody and antigen. |
Antibody First Published | Ball et al. Isolation and characterization of human monoclonal antibodies to digoxin. J Immunol. 1999 Aug 15;163(4):2291-8. PMID: 10438974 |
Note on publication | This article describes the generation and characterisation of human anti-digoxin mAbs raised in transgenic mice with the humanised humoral immune system. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
