Key features and details | |
Cat. No. | MABL-1375 |
Name | Anti-desmoglein-2 mAbs |
Clone No. | AFD- 6D8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes human desmoglein-2. This clone does not cross-react with Desmoglein 1 or Desmoglein 3. The epitope is within the extracellular domain (EC3/EC4) of Desmoglein 2/DSG2. Desmoglein-2 is part of a cadherin protein family and is involved in the creation of desmosomes. |
Alternative Name | Cadherin family member 5; Desmoglein 2; HDGC; DSG2; 3G132 |
UniProt | Q14126 |
Immunogen | This antibody was raised by immunizing mice with a recombinant human full-length DSG2 protein. |
Application Notes | The antibody 6D8 has been used successfully to identify and analyse desmoglein-2 via various methods. For instance, it was utilized to label desmoglein 2 in melanoma tissue samples via immunohistochemistry to investiagete expression pattern of DSG2 in this cancer (Tan et al., 2016; pmid: 27340778). Furthermore, this antibody was also used to label DSG2 during flow cytometric analysis of cells (Tan et al., 2016; pmid: 27340778). The antibody 6D8 has also been applied in a Western blot detection of desmogleins in transfected A-431D cells (Wahl, 2002; pmid: 11991815). Finally, this antibody immunoprecipitated desmoglein-a from A-431 cell extracts prepared in 0.1% Empigen BB (Wahl, 2002; pmid: 11991815). |
Antibody First Published | Wahl et al. Plakoglobin domains that define its association with the desmosomal cadherins and the classical cadherins: identification of unique and shared domains. J Cell Sci. 1996 May;109 ( Pt 5): 1143-54. PMID: 8743961 |
Note on publication | This article describes the characterization of the antibody 6D8. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
