Key features and details | |
Cat. No. | MABL-1367 |
Name | Anti-Deoxynivalenol mAbs |
Clone No. | AFD- AbDON01 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Fusarium species |
Basic Information | |
Specificity | This antibody is specific for mycotoxin deoxynivalenol (DON). This antibody also shows reactivity with other trichothecene mycotoxins like 15-AC-deoxynivalenol, HT-2 toxin, T-2 triol, nivalenol (NIV) and T-2 toxin/tri-acetyl-DON (Tri-Ac-DON) but it does not react with 3-acetyl-deoxynivalenol (3-Ac- DON), Fusarenone-X (FX), trichothecin, diacetoxyscirpenol (DAS), roridin A, and verrucarol. . Deoxynivalenol belongs to the trichothecene class of mycotoxins, generated by several Fusarium fungi. Deoxynivalenol (DON), along with 3-acetyl-deoxynivalenol (3-ADON) and 15-acetyldeoxynivalenol (15-ADON), occur in grains and cereal products and is often hazardous to humans and livestock. Biochemically, deoxynivalenol is an inhibitor of protein synthesis and has hemolytic activity, causing apoptosis (cytotoxicity) and immunotoxicity to eukaryotic organisms. |
Alternative Name | DON; Mycotoxin; Vomitoxin, Fusarium mycotoxin; ChEBI: 10022; 1.3G2 |
UniProt | |
Immunogen | The original antibody was generated by cloning the variable light and heavy chain genes of the anti-DOM hybridoma were assembled into the scFv gene using a linker sequence (Gly4Ser)3 by over- lapping PCR. PCR amplified products were purified, treated with SfiI and NotI and cloned into phagemid expression pDisplay-2TM . The plasmid was used to transform E.coli HB2151 host cells and the soluble scFv was generated. |
Application Notes | The binding characterization of the scFv antibody and the whole IgG1 antibody was done using non-competitive indirect ELISA. To check the cross reactivity of the scFv version and whole IgG version with other members of trichothecene mycotoxins, competitive direct ELISA and biolayer interferometry (BLI) was used (Maragos et al., 2012). |
Antibody First Published | Maragos et al. Production and characterization of a single chain variable fragment (scFv) against the mycotoxin deoxynivalenol. Food and Agricultural Immunology (2012), 23:1, 51-67 |
Note on publication | Describes the generation of a single chain antibody fragment (scFv) antibody having reactivity towards deoxynivalenol. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
